Part:BBa_K1172907:Design

Biosafety-System S (araC)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1374
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1298
  Illegal BamHI site found at 2000
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1416
  Illegal AgeI site found at 1716
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1173
  

Design Notes

Classical [http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#Prefix_Insertion Prefix Insertion] of the first Biosafety-System part BBa_K1172905 into the pSB1C3 backbone of the second part